Capture and Amplification by Tailing and Switching (CATS)

نویسندگان

  • Andrey Turchinovich
  • Harald Surowy
  • Andrius Serva
  • Marc Zapatka
  • Peter Lichter
  • Barbara Burwinkel
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA.

Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of DNA or RNA still remains a major challenge, especially for degraded nucleic acids like circulating DNA. This together with high cost and time requirements impedes many important applications of MPS in medicine and fundamental sci...

متن کامل

A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR

Non-specific tail sequences are often added to the 5'-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in vitro and in silico analysis of the enhancing effect of primer tailing on 2 well-established foo...

متن کامل

Clay sedimentation and consolidation behaviour in tailing storage facilities over mine lifetime

Changes in the tailing properties (increasing clay mineral content and fine particles) and poor operation of the dewatering systems have negative impacts on the Tailing Storage Facilities (TSF) of the Shahrbabak copper complex. The design solid concentration of the thickened tailings is 63 wt.% in the Shahrbabak paste plant but it is well below the design value right now (approx. 55 wt.%). The ...

متن کامل

Parametric Estimates of High Frequency Market Microstructure Noise as an Unsystematic Risk

Noise is essential for the existence of a liquid market, and if noise traders are not present in the market, the trade volume will drop severely and an important aspect of the market philosophy will be lost. However, these noise traders bring noise to the market, and the existence of noise in prices indicates a temporary deviation in prices from their fundamental values. In particular, high-fre...

متن کامل

Controlled ribonucleotide tailing of cDNA ends (CRTC) by terminal deoxynucleotidyl transferase: a new approach in PCR-mediated analysis of mRNA sequences.

Controlled ribonucleotide tailing of cDNA ends (CRTC) by terminal deoxynucleotidyl transferase is a polymerase chain reaction (PCR)-mediated technique that was developed to facilitate cloning and direct sequence analysis of complete 5'-terminal unknown coding regions of rare RNA molecules. In contrast with standard tailing protocols using dNTPs as the substrate, ribo-tailing of cDNA ends is eas...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2014